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. 2000 Oct 31;97(23):12631–12636. doi: 10.1073/pnas.190351397

Figure 2.

Figure 2

V motif is the determinant of the actin polymerization activity of VCA. (A) Schematic structures of chimeric VCAs. (B) Actin polymerization stimulated by chimeric VCAs (100 nM). (C) Binding of G-actin and Arp2/3 complex to wild-type and chimeric VCAs. The GST fusion proteins were tested for their ability to pull-down either G-actin or Arp2/3 complex from solution. Final concentrations of G-actin were 0.06, 0.28, 1.4, and 7 μM, and those of Arp2/3 complex were 2, 10, 50, and 250 nM. The bound proteins were analyzed by Western blotting with anti-actin and anti-Arp3 antibodies.