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. 2000 Oct 31;97(23):12631–12636. doi: 10.1073/pnas.190351397

Figure 3.

Figure 3

Two V motifs of N-WASP VCA are required for its full activity. (A) Schematic structures of N-WASP MV1, MV2, and MVD VCAs. (B) The effect of mutation in V motif on actin polymerization. Actin polymerization was followed in the presence of MV1, MV2, or MVD VCAs (100 nM). (C) The ability of MV1, MV2, and MVD VCAs to bind G-actin and Arp2/3 complex. MV1, MV2, and MVD VCAs were immobilized on beads and incubated with G-actin or Arp2/3 complex. Bound proteins were analyzed by Western blotting.