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. 2000 Oct 31;97(23):12631–12636. doi: 10.1073/pnas.190351397

Figure 5.

Figure 5

Addition of another V motif to WAVE1 VCA increases its activity. (A) Schematic structure of WAVE1 V2CA. (B) The effect of addition of another V motif to WAVE1 VCA on actin polymerization. Actin polymerization was stimulated by WAVE1 V2CA (100 nM). (C) The ability of WAVE1 V2CA to bind G-actin and Arp2/3 complex. WAVE1 V2CA was immobilized on beads and incubated with G-actin or Arp2/3 complex. Bound proteins were analyzed by Western blotting. (D) COS7 cells overexpressing N-WASP/WAVE1 V2CA were stimulated with EGF, and morphological change was quantitated as described in Fig. 4B. (E) Immunofluorescence staining of cells transfected with N-WASP/WAVE1 V2CA. Cells were stained with phalloidin and antibody against WAVE1 VCA.