Figure 1.

Schematic outline of the RaSH protocol. This scheme involves construction of tester (IFN-β + MEZ) and driver (control) HO-1 libraries, followed by digestion of only the tester library with XhoI. After hybridization, differentially expressed sequences are cloned into XhoI-digested vectors, resulting in a subtracted cDNA library enriched for mda genes displaying elevated expression. By using the control HO-1 library as the tester and the IFN-β + MEZ library as the driver, RaSH can also be used to produce a subtracted cDNA library enriched for genes down-regulated during terminal differentiation.