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Reverse Northern blot analysis of differentially expressed sequence tags identified by RaSH. PCR-amplified products from bacterial clones of both RaSH-derived subtracted libraries, Dpn-sLib and EcoR-sLib, were dot-blotted onto nylon membranes and were probed with [32P]cDNA reverse-transcribed from RNA samples of control or IFN-β + MEZ-treated HO-1 cells.
