Figure 3.

Differential expression of representative types of mda genes identified by RaSH and reverse Northern blotting. Northern blots of total RNA were prepared from HO-1 cells untreated (control; lanes 1 and 7) or treated for 2 h (lane 2), 4 h (lane 3), 8 h (lane 4), 24 h (lanes 5 and 10), or 72 h (lane 6) with IFN-β + MEZ, 24 h with IFN-β (lane 8), or 24 h with MEZ (lane 9). Membranes were probed with radiolabeled (³²P) labeled mda sequence tags, identified by RaSH in the Dpn-sLib and EcoR-sLib. Equal loading of samples was confirmed by hybridization with a ³²P-labeled glyceraldehyde-3-phosphate dehydrogenase cDNA probe.