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. 2000 Oct 31;97(23):12684–12689. doi: 10.1073/pnas.220431297

Figure 4.

Figure 4

Determination of the amounts of fibronectin and LIF fragments in PCR cDNA libraries. Relative amount of fibronectin and LIF fragments in PCR libraries was determined by comparison of the signal intensity of fibronectin and LIF in specified cDNA libraries (Dpn-sLib and EcoR-sLib) with the signal intensity of defined amounts of the cDNA fragments by Southern blot hybridization. Lane 1, 100 ng of PCR-cDNA library from untreated cells; lane 2, 100 ng of PCR-cDNA library from IFN-β + MEZ treated cells; lanes 3 to 7, increasing amounts of cDNA fragments of fibronectin and LIF (0.001, 0.01, 0.1, 1, and 10 ng, respectively). PhosphorImager (Molecular Dynamics) scanning determined hybridized signal intensity.