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. 2000 Nov 7;97(23):12694–12699. doi: 10.1073/pnas.97.23.12694

Figure 2.

Figure 2

Partial restoration of ELC but not SLC mRNA expression in spleen of plt/plt mice that have been reconstituted with wild-type bone marrow. (A) Flow cytometric analysis of splenocytes from Ly9.1+ plt/plt mice, Ly9.1− B10D2 wild-type mice (wt), or irradiated plt/plt mice that had been reconstituted with wild-type bone marrow (wt →plt/plt). Left histograms show Ly9.1 on B cells (B220+), and central histograms show Ly9.1 on DCs (CD11c+B220−CD3−). Right histograms display the proportions of CD11c+ cells that express CD8α. Numbers indicate the percentage of cells in the gated population. The data are representative of at least four mice of each type. (B) RT-PCR analysis of ELC and SLC mRNA expression in spleen of wild-type (wt), plt/plt, and bone marrow chimeric mice. Total spleen RNA from the indicated mice was analyzed by RT-PCR with primers specific for ELC-atg, ELC-acg, SLC-ser, SLC-leu, and HPRT. (C) Real-time quantitative RT-PCR analysis of ELC-atg and total SLC expression in samples of the same type as in B. Each dot represents an individual mouse, and bars represent the mean.