Table 1.
Correlation between glycemia and insulitis in double transgenic mice
| Genotype | Histopathology of pancreata
|
||||
|---|---|---|---|---|---|
| Age day | Insulitis
|
Normal (%) | # Islets counted | ||
| P | C | ||||
| (%) | |||||
| Parental strains | |||||
| BALB/c IL-4R −/− normoglycemic | 140 | 0 | 0 | 100 | 55 |
| B10.D2 RIP-HA +/− normoglycemic | 84 | 0 | 0 | 100 | 70 |
| BALB/c TCR-HA +/− normoglycemic | 140 | 0 | 0 | 100 | 68 |
| dTg mice | |||||
| TCR-HA +/−, RIP-HA +/−, IL-4R +/− normoglycemic | 84 | 0 | 0 | 100 | 27 |
| 84 | 0 | 0 | 100 | 20 | |
| 224 | 30 | 61 | 9 | 60 | |
| 224 | 7 | 79 | 14 | 14 | |
| 224 | 66 | 34 | 0 | 5 | |
| TCR-HA +/−, RIP-HA +/−, IL-4R +/− hyperglycemic | 70 | 13 | 83 | 3.6 | 138 |
| 77 | 7.1 | 78.6 | 14.3 | 14 | |
| 98 | 71 | 29 | 0 | 27 | |
| 196 | 5.5 | 94.5 | 0 | 28 | |
| 224 | 60 | 40 | 0 | 5 | |
| TCR-HA +/−, RIP-HA +/−, IL-4R −/− normoglycemic | 42 | 0 | 0 | 100 | 35 |
| 56 | 68 | 0 | 32 | 67 | |
| 56 | 0 | 0 | 100 | 35 | |
| 70 | 0 | 0 | 100 | 26 | |
| 98 | 0 | 0 | 100 | 24 | |
| 182 | 0 | 0 | 100 | 23 | |
| 182 | 0 | 0 | 100 | 22 | |
| 216 | 16 | 0 | 84 | 29 | |
| 216 | 8 | 0 | 92 | 20 | |
| 270 | 50 | 0 | 50 | 22 | |
| 270 | 0 | 0 | 100 | 39 | |
| TCR-HA +/−, RIP-HA +/−, IL-4R −/− | 98 | 71 | 29 | 0 | 42 |
| 140 | 63 | 37 | 0 | 19 | |
| 140 | 75 | 25 | 0 | 28 | |
P, peripheral-insulitis; C, pan-insulitis. Histological analysis of pancreata of eight hyperglycemic 84-day-old TCR-HA +/−, RIP-HA +/− double Tg mice showed 32 ± 21 pan-insulitis.