Table 1.
Purification of the epithelial defensin inducer from yeast extract
Weight, mg | Specific activity, unit/mg | Total units | Yield, % | |
---|---|---|---|---|
Yeast extract | 50 × 103 | 1 | 5 × 104 | 100 |
Acetone precipitate | 2.7 × 103 | 10.67 | 2.88 × 104 | 58 |
Isoleucine | 490 | 50.80 | 2.49 × 104 | 50 |
The defensin-inducing activity was purified as described in Materials and Methods. A unit is defined as the amount of substance that effects peak stimulation of defensin expression.