Table 2.
Quantitative RT-PCR analysis of HOX expression
| Controls | ΔCt
|
||||||
|---|---|---|---|---|---|---|---|
| A1 | A7 | A9 | B9 | A10 | WNT7A | ||
| 302-1 | NHBE | 8.9 | Ab. | Ab. | 16.1 | 15.4 | 4.5 |
| 604-2 | NHBE | 9.8 | Ab. | Ab. | 18.0 | 15.6 | 5.0 |
| 2523 | NHBE | 8.9 | Ab. | Ab. | 15.0 | 15.8 | 4.8 |
| 5214 | T-Ag, p83 | 17.3 | Ab. | Ab. | 15.7 | 15.4 | 16.6 |
| Nonmalignant Lung | 10.8 | 7.7 | 15.3 | 12.9 | 11.6 | 6.1 | |
| HUVEC | Endothel. | 12.8 | 8.7 | 10.1 | 10.0 | 9.1 | Ab. |
| Cell lines | |||||||
| GLC20 | SCLC | 11.1 | 6.8 | 11.1 | 6.8 | 6.9 | 8.2 |
| H187 | SCLC | 11.5 | 7.1 | 9.7 | 9.8 | 7.6 | 19.5 |
| H1048 | SCLC | 10.8 | 7.5 | 9.2 | 10.1 | 7.8 | 15.3 |
| H1341 | SCLC | 12.4 | 15.2 | 8.1 | 8.3 | 10.6 | 15.4 |
| H1607 | SCLC | 9.3 | 13.3 | 18.1 | 6.7 | 15.5 | 5.9 |
| N177 | SCLC | 17.8 | 10.3 | 11.3 | 19.0 | 9.2 | 15.4 |
| H82 | SCLC | 11.7 | 11.1 | 17.6 | 8.4 | 9.9 | 19.9 |
| H196 | SCLC | 8.9 | 12.9 | 19.7 | 11.5 | 6.5 | 19.3 |
| H433 | SCLC | 12.0 | 12.0 | 10.6 | 11.9 | 7.4 | 15.8 |
| H437 | SCLC | 9.1 | 9.9 | 15.5 | 9.2 | 8.4 | 18.8 |
| H1264 | Adeno. | 14.8 | 9.5 | 9.7 | 8.7 | 8.6 | 19.2 |
| H1648 | Adeno. | 8.5 | 12.8 | 20.6 | 11.4 | 15.5 | 10.3 |
| H2122 | Adeno. | 11.4 | 12.4 | 19.6 | 11.0 | 15.0 | 11.5 |
| A549 | Adeno. | 12.4 | 9.7 | 14.3 | 8.4 | 9.4 | 14.6 |
| H460 | Lg. Cell | 14.1 | 10.1 | 9.2 | 6.4 | 9.0 | 20.3 |
| H661 | Lg. Cell | 10.1 | 5.6 | 16.2 | 6.6 | 7.7 | 14.7 |
| H1334 | Lg. Cell | 10.1 | 13.7 | 16.4 | 10.4 | 8.2 | 9.3 |
| H157 | Squam. | 11.5 | 15.5 | 18.8 | 9.5 | 13.1 | 19.9 |
| H226 | Squam. | 11.5 | 15.3 | 14.3 | 9.4 | 6.7 | 18.8 |
| H28 | Meso. | 13.4 | 14.9 | 20.6 | 6.8 | 14.9 | 18.7 |
| H513 | Meso. | 7.8 | 7.2 | 14.6 | 6.7 | 5.9 | 6.2 |
| H290 | Meso. | 8.8 | 12.5 | 12.5 | 8.3 | 6.8 | 9.8 |
| H322 | BronchAlv. | 9.8 | 14.5 | 16.7 | 13.4 | 9.0 | 7.6 |
| H292 | Carcinoid | 9.6 | 12.2 | 9.9 | 11.9 | 7.3 | 6.8 |
| H720 | Carcinoid | 12.0 | 7.4 | 14.9 | 6.4 | 6.9 | 19.4 |
| Direct tumors | |||||||
| tumor03 | Adeno. | 8.6 | 8.8 | 9.1 | 6.8 | 6.1 | 14.5 |
| tumor07 | Adeno. | 14.3 | 13.9 | 12.8 | 16.9 | 16.2 | 15.7 |
| tumor08 | Adeno. | 13.5 | 11.2 | 13.0 | 15.8 | 5.9 | 11.1 |
| 3279-T | Adeno. | 11.0 | 10.0 | 17.7 | 9.5 | 10.1 | 7.4 |
| 3483-T | Adeno. | 12.1 | 10.0 | 16.8 | 9.4 | 8.7 | 8.8 |
| 3662-T | Adeno. | 11.6 | 12.2 | 17.6 | 14.4 | 14.0 | 14.0 |
| 3663-T | Adeno. | 7.9 | 10.6 | 13.4 | 12.2 | 8.3 | 9.0 |
| 3740-T | Adeno. | 10.6 | 9.1 | 16.6 | 13.2 | 8.6 | 9.8 |
| 6160-T | Adeno. | 13.1 | 15.4 | 18.4 | 15.5 | 14.4 | 11.2 |
| 6802-T | Adeno. | 9.5 | 15.5 | 14.6 | 5.7 | 9.0 | 8.1 |
| 7075-T | Adeno. | 13.8 | 16.5 | 18.0 | 15.5 | 14.3 | 13.4 |
| 7257-T | Adeno. | 12.2 | 13.5 | 15.6 | 11.7 | 10.6 | 10.4 |
| 7332-T | Adeno. | 10.4 | 13.7 | 14.3 | 11.8 | 11.8 | 10.6 |
| 7343-T | Adeno. | 14.9 | 16.2 | 18.2 | 12.8 | 13.9 | 11.3 |
| 7345-T | Adeno. | 10.9 | 13.3 | 15.9 | 15.1 | 8.4 | 13.0 |
| 7566-T | Adeno. | 12.5 | 15.0 | 16.4 | 5.4 | 9.2 | 17.0 |
| 2520-T | BronchAlv. | 12.4 | 12.9 | 16.1 | 7.5 | 11.6 | 9.9 |
| 6157-T | Carcinoid | 15.1 | 16.2 | 18.3 | 16.1 | 14.7 | 12.3 |
| 6374-T | Carcinoid | 15.4 | 16.8 | 17.8 | 15.3 | 15.9 | 12.0 |
| 2665-T | Carcinoid | 14.6 | 16.8 | 18.2 | 16.8 | 14.0 | 18.0 |
| 6206-T | Lg. Cell | 8.4 | 12.1 | 16.2 | 8.8 | 7.3 | 15.4 |
| 7362-T | Meso. | 9.4 | 14.3 | 12.7 | 12.3 | 7.2 | 18.0 |
| tumor02 | Squam. | 9.5 | 10.4 | 12.7 | 13.4 | 8.5 | 7.3 |
| tumor11 | Squam. | 12.1 | 10.4 | 12.9 | 12.6 | 8.5 | 12.2 |
| tumor12 | Squam. | 10.3 | 13.2 | 12.9 | 13.5 | 8.6 | 15.8 |
| tumor13 | Squam. | 10.6 | 9.0 | 13.9 | 13.6 | 8.9 | 17.0 |
| tumor14 | Squam. | 11.8 | 10.2 | 17.0 | 15.0 | 8.9 | 10.1 |
| tumor15 | Squam. | 10.3 | 10.0 | 10.5 | 16.1 | 9.4 | 8.2 |
| tumor17 | Squam. | 13.0 | 9.3 | 10.7 | 13.4 | 8.7 | 8.1 |
| 3041-T | Squam. | 11.0 | 8.2 | 16.8 | 13.2 | 12.0 | 9.4 |
| 6406-T | Squam. | 9.6 | 15.8 | 17.0 | 11.0 | 8.8 | 12.5 |
| 6792-T | Squam. | 11.7 | 16.1 | 17.2 | 16.4 | 8.6 | 13.1 |
| 7259-T | Squam. | 10.0 | 12.6 | 12.8 | 9.7 | 9.7 | 12.8 |
| 7466-T | Squam. | 10.6 | 15.2 | 15.4 | 13.7 | 12.4 | 8.0 |
| 7565-T | Squam. | 9.4 | 12.6 | 13.9 | 9.1 | 8.3 | 9.5 |
Values represent ΔCts obtained from 6 controls, 25 lung tumor cell lines, and 35 resected tumors, as indicated. Nonmalignant lung values represent the average of 25 independent resections, which were tumor free. The All HOX column contains the sum of ΔCt values for the 5 HOX loci analyzed. The average standard deviation was 0.12 cycles.