FIG. 1.

Dose response of cPKC inhibitor Gö 6976. Confluent HAEC monolayers were either untreated (A) or treated with 10 μM As(III) (B) for 1 h and the intercellular gaps (indicated with arrows) were visualized by immunofluorescence staining of VE-cadherin (green). The nuclei were stained with Hoechst (blue). Pretreating the cells with increasing concentration of Gö 6976 for 1 h prior to and during 10 μM As(III) treatment inhibited the gap formation in a concentration-dependent manner: 10 μM As(III) + 10 nM Gö 6976 (C), 10 μM As(III) + 50 nM Gö 6976 (D), 10 μM As(III) + 100 nM Gö 6976 (E), 10 μM As(III) + 250 nM Gö 6976 (F), 10 μM As(III) + 500 nM Gö 6976 (G) and 10 μM As(III) + 1 μM Gö 6976 (H). Magnification=60X. The images are representative of 3 independent experiments performed.