FIG. 5.

Effect of As(III) on β-catenin localization. Confluent HAECs were either untreated (A) or treated with 1 μM As(III) (B), 5 μM As(III) (C) and 10 μM As(III) (D) for 6 h and β-catenin localization was visualized by immunofluorescence staining (green). Non-uniform staining or loss of β-catenin was observed wherever gaps occurred (indicated by arrows). Pretreatment with 250 nM Gö 6976 for 1 h prior to and during 10 μM As(III) treatment (E) restored the β-catenin localization at cell-cell junctions. The nuclei were stained with Hoechst (blue). Magnification=60X. The images are representative of 3 independent experiments performed.