FIG. 6.

Effect of As(III) on PY of β-catenin. Confluent HAECs were treated with 1, 5, and 10 μM As(III) for 1 h or pretreated with 250 nM Gö 6976 for 1 h prior to and during 10 μM As(III) treatment. The β-catenin was immunoprecipitated, and Western blotting technique was used to analyze the phosphorylation of tyrosine residues (A). The membrane was stripped and reprobed to determine the total β-catenin protein content (B). The images A and B are representative of 4-6 independent experiments performed. The percent change in PY with the various treatments relative to the control (untreated) is represented in (C). The control (100%) is indicated by the dotted line. Error bars represent mean ± SE for n=4-6. * and # indicates significantly different from control and 10 μM As(III), respectively, with p < 0.01.