Table 3.
Twenty most often positively selected rbcL residues
| Residue No 1 | N 2 | Location of residue | Residues within 5 Å 3 | Structural motifs within 5 Å | Interactions4 |
| 251 | 46 | helix 3 | 247, 248, 249, 250, 252, 253, 254, 255, 256, 279, 280, 283 | helixes 3, 4 | DD, SSU |
| 225 | 39 | helix 2 | 189, 190, 193, 194, 221, 222, 223, 224, 226, 227, 228, 229, 236, 237, 238 | helixes 1, 2; strand 3 | SSU |
| 142 | 31 | helix D | 33, 140, 141, 143, 144, 145, 146, 367, 369 | N-terminus; strands D, H | DD |
| 328 | 28 | loop 6 | 295, 311, 326, 327, 329, 330, 342, 345, 346, 349, 376, 377, 378, 394 | AS; loop 6 region; helixes 5, 7; strand 7 | AS |
| 449 | 28 | helix G | 445, 446, 447, 448, 450, 451, 452, 453, 455, 456 | C-terminus | SSU |
| 145 | 27 | helix D | 140, 141, 142, 143, 144, 146, 147, 148, 320, 366, 367, 368, 369, 371 | helixes D, 5, H | DD |
| 86 | 23 | strand C | 25, 27, 84, 85, 87, 88, 98, 99, 100 | strands A, C, D | RA |
| 309 | 22 | strand F | 117, 121, 125, 134, 135, 301, 302, 307, 308, 310, 311, 313, 314 | strand E; helixes F, 5 | ID |
| 95 | 21 | 42, 43, 44, 93, 94, 96, 97, 131 | strands B, D, E | ID, RA | |
| 375 | 19 | strand 7 | 155, 158, 159, 169, 324, 325, 326, 373, 374, 376, 377, 397, 398, 399 | helix E; strands 6, 7, 8 | SSU |
| 470 | 19 | C-terminus | 336, 468, 469, 471, 472 | loop 6; C-terminus | ID, RA |
| 279 | 18 | helix 4 | 250, 251, 274, 275, 276, 277, 278, 280, 281, 282, 283 | helixes 3, 4 | |
| 219 | 17 | helix 2 | 58I, 59I, 61I, 214, 215, 216, 217, 218, 219, 220, 221, 222, 223, 256, 260 | helixes 2, 3 | SSU, DD |
| 255 | 17 | helix 3 | 251, 252, 253, 254, 256, 257, 258, 259, 283 | helixes 3, 4 | SSU, DD |
| 28 | 15 | N-terminus | 25, 26, 27, 29, 30, 84 | strands A, C | |
| 228 | 14 | helix 2 | 190, 193, 194, 224, 225, 226, 227, 229, 230, 231, 232, 236 | helixes 1, 2 | SSU |
| 97 | 13 | strand D | 40, 41, 42, 44, 50, 87, 88, 89, 90, 95, 96, 98, 99, 100 | helix B; strands B, C, D | RA |
| 262 | 12 | 59S, 60S, 226, 240, 257, 258, 260, 261, 263, 264, 289 | helixes 2, 3; strand 3 | SSU, DD | |
| 439 | 12 | helix G | 436, 437, 438, 440, 441 | helix G | |
| 282 | 10 | helix 4 | 149, 278, 279, 280, 281, 283, 284, 285, 286, 321 | helixes 4, 5 | DD, SSU |
1 Numbering of residues is after the spinach Rubisco sequence.
2 Number of groups with detected signal of positive selection where the particular residue was shown under positive selection with Bayesian posterior probability larger than 0.95, when analyzed by the Bayes Empirical Bayes [20].
3 Subscriptions denote residues from I and S small subunits. Residues within the list of the twenty designated residues are underlined.
4 Interactions in which the twenty selected residues and/or residues within 5 Å of them are involved. AS – interactions with the active site; ID – intradimer interactions; DD – dimer-dimer interactions; RA – interface for interactions with Rubisco activase; SSU – interactions with small subunits; after [8]. Interactions based on literature survey only are given in italics; after [1,23,27].