Table 5.
Sequences for primers and probes for candidate reference genes used in the study (5'-3' direction).
| Gene | Forward Primer | Reverse Primer | Probe | Amplicon Length Bp | Efficiency of Assay (%age) | Accession Number for sequence used |
| GAPDH | CTGGGGCTCACTTGAAAGG | CAAACATGGGGGCATCAG | CTGCTCCT | 72 | 102.3 | NM_001003142 |
| ACTB | CCCATCTACGAGGGGTACG | GGATCTTCATGAGGTAGTCAGTCA | CTGGCTGG | 88 | 101.9 | NM_001003349 |
| B2M | CCTTGCTCCTCATCCTCCT | TGGGTGTCGTGAGTACACTTG | CAGCATCC | 83 | 99.5 | XM_535458 |
| HMBS | GCATGTATGCTGTGGGTCAG | GCATGTCCTGGTCCTTGG | GGCCCTGG | 63 | 101.0 | XM_546491 |
| HPRT | TGTGCTCAAGGGAGGCTATAA | CCATCACTATTTCTGTTCAGTGCT | ACCTGCTG | 75 | 101.8 | NM_001003357 |
| RPL13A | CTGCCCCACAAGACCAAG | GGGATCCCATCAAACACCT | CCAGGCTG | 65 | 98.6 | ENSCAFG00000003633 |
| SDHA | GGTGGCACTTCTACGACACC | ATGTAGTGGATGGCGTCCTG | CTGGCTGG | 67 | 102.5 | XM_535807 |
| TBP | TCCACAGCCTATCCAGAACA | CTGCTGCTGTTGTCTCTGCT | CTGGAGGA | 67 | 97.4 | ENSCAFG00000004119 |
| YWHAZ | GTTACTTGGCCGAAGTTGCT | TGCTTGTTGTGACTGATCCAC | TGGTGATG | 65 | 100.7 | XM_532287 |