Table 1.
a Host cell | Insertion construct | b Cre | Drug Selection | c Colonies | d GFP-neg | e Tet | f PCR/SB | g I |
Rht14-19 | pIN-1 | + | hyg | 53 | 47/48 | 47/48 | 10/10 | 10 |
Rht14-19 | pIN-1 | - | hyg | 0 | - | - | - | 0 |
Rht14-10 | pIN-2 | + | MPA/X | 11 | 9/11 | 9/11 | 9/9 | 1.8 |
Rht14-10 | pIN-2 | - | MPA/X | 0 | - | - | - | 0 |
Rht14-19 | pIN-2 | + | MPA/X | 32 | 22/33 | 22/33 | 9/9 | 4.4 |
Rht14-19 | pIN-2 | - | MPA/X | 0 | - | - | - | 0 |
Rht14-10 | pIN2-neoMCS | + | G418 | 188 | 181/188 | NT | NT | 36 |
Rht14-10 | pIN2-neoSCE | + | G418 | 169 | 163/169 | 3/3 | 3/3 | 33 |
Rht14-10 | pIN2-neoR52 | + | G418 | 226 | 217/226 | 3/3 | 3/3 | 43 |
a Each row represents data for the transfection of 5 × 105 cells.
b Indicates whether pMC-Cre was (+) or was not (-) co-transfected.
c Total number of drug-resistant colonies generated.
d Colonies that did not express GFP.
e GFP-negative colonies that became drug-sensitive on addition of tet.
f GFP-neg/Tets colonies with expected structure detected by PCR or Southern Blot.
g Cre-mediated insertion frequency expressed as events per transfected cell × 105 (assuming all GFP-neg clones are correctly inserted).