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. 2007 May 10;8:30. doi: 10.1186/1471-2199-8-30

Table 2.

Frequencies of G418r colony formation in pDRneo-transfected cells

Cells Frequency of G418r Fold Induction
+ tet - tet
a 10IN-SCE.1flp1/DRneo, Clone 1 4.7 × 10-6 7.2 × 10-2 1.5 × 104
a 10IN-SCE.1flp1/DRneo, Clone 2 1.3 × 10-6 2.9 × 10-2 2.2 × 104
a 10IN-SCE.1flp1/DRneo, Clone 3 1.0 × 10-6 3.5 × 10-2 3.5 × 104
b Rht14/DRneo 1.3 × 10-5 1.4 × 10-5 1.1

a Three independent pDRneo-transfected 10IN-SCE.1flp1 clones were grown continuously with tet in the medium (+tet) or tet was withdrawn for 48 h. (-tet) prior to G418 selection (methods).

b A single hygror clone, isolated after the transfection of Rht14 cells with pDRneo, was grown for 48 h. and then selected in G418, with tet either present (+tet) or not present (-tet) in the medium throughout (methods).