Table 2.
Frequencies of G418r colony formation in pDRneo-transfected cells
Cells | Frequency of G418r | Fold Induction | |
+ tet | - tet | ||
a 10IN-SCE.1flp1/DRneo, Clone 1 | 4.7 × 10-6 | 7.2 × 10-2 | 1.5 × 104 |
a 10IN-SCE.1flp1/DRneo, Clone 2 | 1.3 × 10-6 | 2.9 × 10-2 | 2.2 × 104 |
a 10IN-SCE.1flp1/DRneo, Clone 3 | 1.0 × 10-6 | 3.5 × 10-2 | 3.5 × 104 |
b Rht14/DRneo | 1.3 × 10-5 | 1.4 × 10-5 | 1.1 |
a Three independent pDRneo-transfected 10IN-SCE.1flp1 clones were grown continuously with tet in the medium (+tet) or tet was withdrawn for 48 h. (-tet) prior to G418 selection (methods).
b A single hygror clone, isolated after the transfection of Rht14 cells with pDRneo, was grown for 48 h. and then selected in G418, with tet either present (+tet) or not present (-tet) in the medium throughout (methods).