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Western blot analysis of shake flask and bioreactor batch cultivation samples. Western blots using anti-ROL antibodies (A) and anti-GFP antibodies (B). Lane 1: ROL positive control, lane 2: culture supernatant from clone GFP-ROL 1.5.5; lane 3: culture supernatant from clone ROL-GFP 2.15.2; lane 4: culture supernatant from bioreactor culture of clone GFP-ROL 1.5.5.
