Table 2.
Sequences of cytochrome P450 2E1 and Multidrug Resistance-1 mRNA forward and reverse oligonucleotide primers and sequence of crs-primers for synthesizing internal competitive standard.
Gene | Oligonucelotide sequence (5′-3′) | Location | |
---|---|---|---|
CYP2E1 | Forward | AGC ACA ACT CTG AGA TAT GG | 925-944 |
Reverse | ATA GCT ACT GTA CTT GAA CT | 1271-1290 | |
crs-CYP2E1 | Forward | CAT TTA ATA CGA CTC ACT ATA GGG ACC TAC ATG GAT GCT GTG GTG | T7 polymerase sequence |
Reverse | TAT AGG GCT GAG GTC GAT ATC CTT CAC ACT CGT TTT CCT GTG GA | 1432-1413 & 1409-1396 |
*NIDDM volunteer was successfully controlling their diabetes with dietary modification. †Female volunteer, African American volunteer. ‡Glycosylated haemoglobin normal range 4.8–8.0%.