Figure 2.

Agarose gel stained with ethidium bromide showing the 147-bp amplified Y. pestis pla fragment obtained from ancient DNA after suicide PCR. Products resulting from DNA extracted from the dental pulp tissues collected from the ancient child skeletons (lanes 3, 5, 8, and 10), negative control dental pulp tissues (lanes 4, 9, 11, and 12), and mock extraction controls (lanes 2, 6, and 7) are shown. Lanes 1 and 13 are molecular weight marker fragments. The measured molecular weight of the amplicon is indicated in the margin.