TABLE 1.
The Effect of Receptor Tyrosine Kinases on Shaker Current Properties
| Channel Construct and Co-transfected Kinase | Peak Current (pA) | τInact (ms) | τDeact (ms) |
|---|---|---|---|
| WT Kv1.3 (n= 23) | 600 ± 71 | 739 ± 105 | 15.5 ± 1.6 |
| WTKv1.3 +TrkB (n=33) | *724 ± 63 | 668 ± 44 | 16.9 ± 1.4 |
| WTKv1.3 + IR (n=11) | 653 ± 55 | 737 ± 64 | 15.4 ± 1.2 |
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| |||
| WTKv1.5 (n=20) | 690 ± 82 | 108 ± 12 | 8.8 ± 1.3 |
| WTKv1.5 +TrkB (n=11) | 682 ± 84 | 104 ± 10 | 9.1 ± 1.2 |
| WTKv1.5 +IR (n=10) | 634 ± 133 | 83 ± 6 | 7.7 ± 1.1 |
HEK 293 cells were transfected with cDNAs encoding Kv1.3 or Kv1.5 and co-transfected with either TrkB receptor or IR kinase. Cells were held in the cell-attached configuration and stepped from -90 mV to +40 mV for 1000 msec using a 45 s interpulse interval. Peak current amplitudes were acquired approximately 5 minutes after achieving seal formation. Mean values (± s.e.m.) for the inactivation time constant (τInact), deactivation time constant (τDeact ), and peak current amplitude were compared across transfection conditions. Data for each respective channel (subfamily member 1.3 or 1.5) were analyzed by completely randomized one-way ANOVA with Student-Newman-Keuls’ (SNK) followup test with significance (*) determined at the 95% confidence level (α ≤ 0.05).