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. 2000 Nov 7;97(23):12816–12821. doi: 10.1073/pnas.97.23.12816

Figure 3.

Figure 3

The expression of 25-Dx is sexually dimorphic in the PRKO mouse hypothalamus. Northern blots were used to examine the expression of 25-Dx in the hypothalamus of male and female PRKO mice. (a) The expression of 25-Dx in the hypothalamus of female PRKO (ko) mice is higher than in wild-type (wt) littermates. The 25-Dx probe hybridizes to a single 1.9-kb transcript in the mouse hypothalamus (Top). The ethidium bromide-stained gels demonstrate the equal loading of RNA across lanes for the Northern blot (Middle) and the corresponding genotype of each animal, as determined by PCR (Bottom). (b) Quantitative analysis of Northern blots. Blots similar to the one shown in a were prepared for male (+/+, n = 3; +/−, n = 12; −/−, n = 14) and female (+/+, n = 12; +/−, n = 13; −/−, n = 14) PRKO mice and quantified by PhosphorImager analysis. *, P < 0.05 (two-tailed), **, P < 0.001 (two-tailed).