Effect of KA injection on neuronal viability in hippocampi from
wt and PS1 tg mice. (Upper) Representative micrographs
of CA3 hippocampal region from wt FVB/N (A and
C) and PS1 L286V tg (B and
D) mice treated with saline (A and
B) or 30 mg/kg KA (C and
D) and killed 3 days after the lesion.
(E) Quantitative analysis of KA-induced cell loss in the
CA3 and dentate gyrus (DG) hippocampal region of wt FVB/N and PS1
L286V tg mice treated with either 15 mg/kg or 30 mg/kg KA and
killed after 3 days (3d) or 6 days (6d), as indicated. Viable cells
were evaluated by estimating the mean profile number per
mm2. Data are expressed as the percent of the corresponding
control values (black bar) and represent the mean ± SEM of at
least 15 sections per each brain. Absolute values were 7.5 ±
0.9 × 105 and 6.9 ± 1.3 × 105
profile number per mm2 in dentate gyrus of wt and PS1 tg
mice, respectively, and 2.1 ± 0.4 × 105 and
2.5 ± 0.5 × 105 profile number per
mm2 in CA3 region of wt and PS1 tg mice, respectively.
*, P < 0.05 vs. the corresponding values of
wt group.