Table 1.
Summary of analytical methodology performance for morphine, morphine 3- and 6-glucuronides (M3G, M6G, respectively), oxycodone and their N-demethylated metabolites, normorphine and noroxycodone, in plasma
| Drug | Mor | Nmor | M3G | M6G | NM3G | IS-Hmor | Oxy | Noxy |
|---|---|---|---|---|---|---|---|---|
| Mrm signal | 286/201 | 272/209 | 462/286 | 462/286 | 447/272 | 286/185 | 316/299 | 302/277 |
| Recovery (%) | 74 ± 7 | 68 ± 4 | 58 ± 4 | 40 ± 10 | 30 ± 4 | 37 ± 4 | 53 ± 3 | 51 ± 1 |
| Calibration range plasma concentration (mg l−1) | 0.078–5 | 0.039–2.5 | 0.156–10 | 0.039–2.5 | 0.039–2.5 | – | 0.078–5 | 0.078–2.5 |
| LOQ (pg on column) | 156 | 78 | 313 | 78 | 78 | – | 156 | 156 |
Mor, Morphine; Nmor, normorphine; M3G, morphine 3-glucuronide; M6G, morphine 6-glucuronide; IS-Hmor, internal standard-hydromorphone; Oxy, oxycodone; Noxy, noroxycodone; Mrm, m/z (mass : charge) ratio of the parent ion to the daughter ion; LOQ, limit of quantification.