Table 1B.
Re-amplification reactions performed for CYP2D6 genotype determination
| CYP2D6 allele (mutation) | Specific primers | Restriction enzyme | Restriction pattern (bp) | Agarose gels, % |
|---|---|---|---|---|
| *2 (C2938T) | F: 5′-AGGCCTTCCTGGCAGAGATGGAG-3′ R: 5′-CCCCTGCACTGTTTCCCAGA-3′ | cfo I | wt:260, 126 mut: 386 | 2.0 |
| *4 (G1934A) | F: 5′-TGCCGCCTTCGCCAACCACT-3′ R: 5′-CTCGGTCTCTCGCTCCGCAC-3′ | Bst NI | wt:292 mut: 111, 181 | 1.5 |
| *10 (G4286C) | F: 5′-GAGACAAACCAGGACCTGCCA-3′ R: 5′-GCCTCAACGTACCCCTGTCTC-3′ | Bst EII | wt:860 mut: 240, 620 | 1.8 |
| *17 (1111C) (wt) | F: 5′-CCAAGGTTCAAATAGGACTA-3′ | wt: 237 | 1.5 | |
| *17 (1111T) (mut) | F: 5′-CCAAGGTTCAAATAGGACTA-3′ R: 5′-CCCGAAACCCAGGATCTGGA-3′ | mut: 237 | 1.5 |
wt, Wild type; mut, mutant; F, forward primer; R, reverse primer.