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. 2000 Oct 24;97(23):12840–12845. doi: 10.1073/pnas.220410397

Figure 3.

Figure 3

Effect of PKA activation on DARPP-32 phosphorylation at Thr-34 and Thr-75 in neostriatum. (a) Immunoblots showing the levels of phospho-Thr-34, phospho-Thr-75, and total DARPP-32 in response to treatment with 10 μM forskolin (FSK), 10 μM 1,9-dideoxyforskolin (dFSK) (an inactive analog of forskolin), or 5 mM 8-bromo-cAMP (cAMP) for 5 min, detected on the same nitrocellulose membrane. (b and c) The amounts of phospho-Thr-34 DARPP-32 (b) and phospho-Thr-75 DARPP-32 (c) were quantified by densitometry, and the data were normalized to values obtained with untreated slices. Data represent means ± SEM for 3–10 experiments. *, P < 0.05; **, P < 0.01 compared with control, Student's t test.