Table 1.
Primer sequences and PCR conditions of the Pyrosequencing™ assays
| Identification* | Forward primer (5′-3′) | Reverse primer (5′-3′) | Internal primer (5′-3′) | PCR thermoprofile |
|---|---|---|---|---|
| rs736342 | ATCCCATGATCATCTCCCTTC | †CTGGCACCAGGATATGTGTG | GCTAAAGTTTCAATGCCT | 95 5′, 45(95 30 s, 62 30 s, 72 30 s) 72 5′, 4 for ever |
| rs2276300 | †CTCCCAGCCTAATCCCTTAC | AACTGAAGCCATGCTGACAC | TGAGACTTCCCATGATAAC | 95 5′, 45(95 15 s, 58 30 s, 72 15 s) 72 5′, 4 for ever |
| rs10897312 | †GCTTCAGAGAATCCCCTTCC | GAAGATGGGGGCCTTTGTTA | AAAAGAAAAATGCTCTCAG | 95 5′, 45(95 15 s, 58 30 s, 72 15 s) 72 5′, 4 for ever |
| rs955434 | GGAGTCCCTGCTTAGAGACAGA | †CCTTCTCTCCATTGCTGCTTAT | CGGAGCTGGGGTT | 95 5′, 45(95 15 s, 60 30 s, 72 15 s) 72 5′, 4 for ever |
| rs953894 | †GATGATAGGGGCTGCTTGAG | TACTGCCTCCTTTGGTCTCC | CACGTCAGACATGACCA | 95 5′, 45(95 15 s, 60 30 s, 72 15 s) 72 5′, 4 for ever |
| OAT3_goe2 | ACTGGCTGTTGATGTTCTTCC | †CTGAGCCTTTCTCCCTCTTC | TCTTGTCTGGAAAGTCCT | 95 5′, 45(95 15 s, 60 30 s, 72 15 s) 72 5′, 4 for ever |
| rs2276299 | †GAGGAGAGGGCCACATACC | GCTACACCTTTGGCCAGTTC | CGTTGGCTGCAGTT | 95 5′, 45(95 15 s, 60 30 s, 72 15 s) 72 5′, 4 for ever |
| rs2187383 | †TGCATTCCTGGATCCCTAGA | ATTGGCCCCAGAACACATAG | CATCCTCTCTGGTCCTT | 95 5′, 45(95 15 s, 60 30 s, 72 15 s) 72 5′, 4 for ever |
| OAT3_goe1 | †ATACCTCTCCCTGGCCTATG | AAGATCTGCAGCAGGTTGTG | GGGAGGCCCAGTATG | 95 5′, 45(95 15 s, 60 30 s, 72 15 s) 72 5′, 4 for ever |
| rs948979 | TAGTGTTAGGGCACACCCAGA | †ATTGAGGGTGGGATGGAAG | CCAGACAGAAAATGTCACT | 95 5′, 45(95 15 s, 60 30 s, 72 15 s) 72 5′, 4 for ever |
| rs3759053 | †CTCCAGCACCAAAAGTGAAAG | AGCCCAGTTGATATTTCTTCCA | TTCAGTAACATCAAAGCAC | 95 5′, 45(95 15 s, 58 30 s, 72 15 s) 72 5′, 4 for ever |
| rs3782099 | †CTGCCTGGTGAGAGGAGA | ACACTCACCTGGCTCTTCC | CACAGCATCCCCTTG | 95 5′, 45(95 15 s, 58 30 s, 72 15 s) 72 5′, 4 for ever |
| rs1783811 | CCGAGGAGTACCACTGTGT | †GGTAAGCCAAGTGTTAGCTC | AAGGAACAAGCCCAA | 95 5′, 45(95 15 s, 58 30 s, 72 15 s) 72 5′, 4 for ever |
| rs4963326 | †TCCCCATCTGAGAGAGAGAATT | GCAGAGGCAGGATTTGAATTTA | CCTTCCTGAACACACTG | 95 5′, 45(95 15 s, 58 30 s, 72 15 s) 72 5′, 4 for ever |
| rs10792369 | CTTTCTGTGGGCCATCAAC | †ACGGGGCCTCAGATATACAA | CCCTCCATCCAATGT | 95 5′, 45(95 15 s, 58 30 s, 72 15 s) 72 5′, 4 for ever |
| rs11231809 | †TAACTCACCTGTTCCGTGATT | ATCAGACGTATCGTTTGTAAGG | ATCGTTTGTAAGGACTCA | 95 5′, 45(95 15 s, 58 30 s, 72 15 s) 72 5′, 4 for ever |
| rs4930423 | †TCCAGGAAACTCCTTTGTCAT | AGATTGAATAATGAGGGGAATACA | ATGAGGGGAATACAGTG | 95 5′, 45(95 15 s, 58 30 s, 72 15 s) 72 5′, 4 for ever |
| rs2078267 | †AGCCCAACAACATCAGAGTA | ACCCAGTTGTTATCTGTCCA | AGGCTCCAGTGTCGG | 95 5′, 45(95 15 s, 58 30 s, 72 15 s) 72 5′, 4 for ever |
| rs528211 | TTAGGGAGGTGTGGCAAGTT | †GGCAAAATCTCATCACAGACC | TTTGAAGACAGAGAGGC | 95 5′, 45(95 15 s, 58 30 s, 72 15 s) 72 5′, 4 for ever |
| rs476037 | GTTAAGTGGAGTCGGTCAGG | †TCAGCTCCACAGCTTTACC | GCAGGCAGAGGATGT | 95 5′, 45(95 15 s, 58 30 s, 72 15 s) 72 5′, 4 for ever |
*
Rs number as in the NCBI database SNP. Two putative polymorphisms, identified when comparing genomic databases, had no reference number (OAT3_goe 1 and 2).
†
Biotinylated primer.