Figure 5.

Human NSCs (hNSCs) possess tumor tracking characteristics. (A and B) Rodent CNS-1 glioblastoma cells and human NSCs were implanted as per Paradigm 3 into opposite hemispheres of an adult mouse. Pictured 7 days later at low power (A) and high power (B) is a section through the neutral red-stained tumor (outlined by arrowheads) intermixed with human NSCs (identified by their brown nuclei following reaction with an anti-human nuclear antibody) (arrows) that migrated from the contralateral side. (C–E) Human HGL21 glioblastoma cells and hNSCs were similarly implanted into opposite hemispheres. Pictured at progressively higher power are sections through that neutral red-stained tumor intermixed with human NSCs (X-Gal⁺ blue) that migrated from the contralateral side. (Scale bars: A, 20 μm, 15 μm in B; C, 60 μm, 30 μm in D, and 15 μm in E.)