Table 2. Effects of selected alleles of genes on mis-reading of non-cognate and near-cognate mutations at codon 218 of firefly luciferase.
| Non-cognate (UCU) | Near-cognate (AGC) | |||||
| Strain | mis-incorp (×10−4) | Fold WT | P-value | mis-incorp.(×10−4) | Fold-WT | P-Value |
| eEF1A | ||||||
| WT (TEF2) | 2.04±0.13 | 1.00 | 1.00 | 7.01±0.49 | 1.00 | 1.00 |
| E40K (TEF2-3) | 1.91±0.18 | 0.94 | 0.56 | 8.11±0.72 | 1.16 | 0.22 |
| E122K (TEF2-4) | 2.33±0.17 | 1.14 | 0.17 | 15.4±0.55 | 2.20 | 9.76E-10 |
| E122Q (TEF2-10) | 1.73±0.16 | 0.85 | 0.14 | 14.4±0.79 | 2.05 | 2.43E-6 |
| D130N (TEF2-13) | 1.61±0.12 | 0.79 | 0.02 | 8.16±0.21 | 1.16 | 0.05 |
| T142I (TEF2-7) | 1.62±0.14 | 0.79 | 0.03 | 4.20±0.48 | 0.60 | 6.64E-4 |
| N153T (tef2-19) | 2.05±0.04 | 1.00 | 0.95 | 6.39±0.14 | 0.91 | 0.06 |
| N153T/D156E (tef2-18) | 1.87±0.05 | 0.91 | 0.03 | 5.53±0.20 | 0.79 | 4.92E-3 |
| D156N (tef2-17) | 1.85±0.04 | 0.91 | 0.02 | 11.94±0.39 | 1.69 | 3.10E-7 |
| E286K (TEF2-1) | 1.81±0.08 | 0.89 | 0.04 | 24.64±0.11 | 3.50 | 3.58E-8 |
| E295K (TEF2-9) | 2.00±0.20 | 0.98 | 0.87 | 10.9±0.95 | 1.55 | 3.09E-3 |
| E317K (TEF2-2) | 2.31±0.04 | 1.13 | 0.06 | 20.1±0.91 | 2.87 | 1.70E-7 |
| eEF1Bα | ||||||
| WT | 3.15±0.12 | 1.00 | 1.00 | 3.50±0.14 | 1 | 1.00 |
| K120R S121Δ I22Δ | 2.38±0.22 | 0.76 | 8.89E-3 | 2.98±0.10 | 0.85 | 0.01 |
| eEF1Bγ | ||||||
| WT | 1.22±0.14 | 1.00 | 1.00 | 1.24±0.09 | 1 | 1.00 |
| tef3Δ | 1.00±0.04 | 0.81 | 0.15 | 1.18±0.05 | 0.95 | 0.49 |
| tef4Δ | 1.25±0.14 | 1.02 | 0.41 | 1.14±0.06 | 0.92 | 0.41 |
| tef3Δ tef4Δ | 1.17±0.05 | 0.96 | 0.62 | 1.62±0.03 | 1.31 | 1.09E-5 |