Table 1.

Primers used for qPCR verification

All primers are listed from 5′ to 3′. Fluorescently labeled primers have a 6-FAM fluor on the 5′ end and a TAMRA fluor on the 3′ end.

Gene	Forward	Reverse	Fluorescently Labeled
17bdhs	TTCCTACGATGCCTCCTTTAGAA	AGTGTAGCGGCCTATGATGGA	CAGAGTACGCTGCCTGGCCCACC
Beta Actin	ACGGCCAGAGGCGTACAG	TTCAACCCTGCCATGT	ACAACACGGCCTGGATGGCCA
Cyt oxidase II	GAGGCAATAAAGGCTGTTTGGT	GAGCCGTTCCTTCTTTAGGTGTAA	TAATCGTCCTGGAACTGCGTCTATC
Estrogen Receptor	GCAGGACCAAACTCCGTAGTG	TGGCCAACGCGAGGTA	TACCCAGAGGCAAAGTCGCTGCAGA
Pyruvate Kinase	TGGTGGGCCTGTCTGTCA	CAGTCCACGTGCCCTAAACA	TCAGTGGTAGTGTCCAATAGGAGCAGA
Retinol Binding Protein	TCTGTATTTGCCGAGGAAGCA	AGGCCCGAAGACCAGAAGA	ATCTCCTTTTTCTTGTTTGTGACA
Serum Albumin 2	TCTTCCCAGAGAGCAGCAGATC	GATGCCAGCAGCTTCCACAT	TTGCTGTCCTTGGTGCAGAGCTCG
Vitellogenin	CTTGTGAACCCTGAGATC	GCAGCTGGGACGAAAGG	TTGAGTACAGTGGTGTGTGGCCCAAAGA
Vitelline Envelope	GCCGGTTCCTCCTCCAAAT	TCCGCTGCCCAGTCTGA	CTGATATAGCTCCTGGGCCCCTCATAGTTG
VSVH induced protein	CGAGACCACGTCGCTCAAT	TGAAAAGATCTCTGTGCGCAAA	TAGACAGTGCTAACAGGCTATTTTGCT