Figure 4. In Vitro Binding of TFL2/LHP1 to H3K27me3, H3K9me3, and H3K9me2.
In vitro–translated and 35S-labeled TFL2/LHP1 was pulled down with either H3K9me2-, H3K9me3-, H3K27me3-, or H3-biotinylated peptides, or without peptide (beads). Purified protein was separated on SDS-PAGE and analyzed by autoradiography. Fractions of the input are shown as indicated. A representative autoradiogram of three independent experimental repetitions is shown.
