Fig 5 Skin biopsy taken at the distal leg in a healthy subject. Double staining confocal microscopy with antibodies against protein gene product 9.5 (green), which stain nerve fibres, and antibodies against collagen IV (red), which stain the dermal-epidermal junction (asterisk) and blood vessels (BV). Arrows indicate intraepidermal nerve fibres and arrowheads indicate dermal nerve bundles. Note the branched intraepidermal fibre arising from a dermal nerve fibre (original magnification ×40)