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Primary antibodies.
| marker | specificity | manufacturer | pre-treatment | dilution |
|---|---|---|---|---|
| S100 | rabbit anti-bovinea | Dako (Denmark) | 0.1% trypsin | 1 : 2500 |
| desmin | mouse anti-humana | Dako | 3×5 min microwave (600 W) | 1 : 100 |
| myogenin | mouse anti-rat | Dako | 3×5 min microwave (600 W) | 1 : 25 |
| PCNA | mouse anti-rat | Dako | 3×5 min microwave (600 W) | ready to use |
Cross-reactivity with rat tissue confirmed by the manufacturer.
