A. Lysates from MCF-7 cells treated with 100nM 1,25(OH)2D3 or ethanol vehicle for 72 hours were subjected to western blotting with an antibody directed against Grim-19. Western blots were scanned and expression of Grim-19 was normalized for protein loading. Data are expressed as mean ± standard error of triplicates. *p<0.05, control vs. 1,25(OH)2D3 treated.
B. Comparison of basal Grim-19 expression in MCF-7 cells and MCF-7DRES cells, which were selected for resistance to 100nM 1,25(OH)2D. Data was obtained as described in A.