Table 1. Identification of 1,25(OH)2D3 regulated proteins in MCF-7 cells.
MCF-7 cells were treated for 72 hours with either ethanol vehicle control (Con) or 100nM 1,25(OH)2D3. Each sample was analyzed in triplicate for expression of 270 apoptosis related proteins. Relative expression of each protein was normalized to several housekeeping genes. The normalized expression of each of the ten proteins listed was significantly different between vehicle and 1,25(OH)2D3 treated cells (p<0.05 by Students t test). Sign of change indicates whether the protein was up or down-regulated by 1,25(OH)2D3 in comparison to control.
| MCF-7 cells Con vs 1,25(OH)2D3 | Sign of Change | Function/Pathway |
|---|---|---|
| Grim19 | − | Mitochondrial oxidoreductase involved in electron transport, linked to apoptosis mediated by interferon β and retinoic acid. |
| Cyclin A | − | Cell cycle regulator that promotes both G1/S and G2/M transitions |
| Cyclin D3 | − | Cell cycle regulator required for G1/S transition |
| Stat 6 | − | Transcription factor activated by cytokine signaling through membrane receptors. Mediates anti-apoptotic activity of IL-4. |
| GSK-3b | − | Proline-directed serine-threonine kinase with multiple substrates, including β-catenin. |
| Integrin linked kinase(ILK) | − | Serine-threonine kinase, mediates integrin signaling to cell survival through AKT pathway |
| Rho-GDI | − | GDP dissociation inhibitor, stimulates signaling through Rho, a GTPase that regulates focal adhesions and stress fibers |
| SHC | − | Adaptor molecule in ras signal transduction |
| Cathepsin D | + | Lysosomal protease implicated in caspase independent cell death via autophagy |
| PTEN | + | Protein/lipid phosphatase that preferentially dephosphorylates phosphoinositides. Negative regulator of AKT. |