Table 2. Identification of proteins differentially expressed in MCF-7 versus MCF-7^DRES cell.

Lysates from MCF-7 and MCF-7^DRES cells were analyzed in triplicate for expression of 270 apoptosis related proteins. Relative expression of each protein was normalized to several housekeeping genes. The normalized expression of the fourteen proteins listed were significantly different between MCF-7 and MCF-7^DRES cells (p<0.05 by Students t test). Sign of change indicates whether the protein was up or down-regulated in MCF-7^DRES cells relative to MCF-7 cells.

Protein	Sign of Change	Function/Pathway
ACAP2	+	GTPase-activating protein, activates ARF6, a regulator of cell adhesion and motility
CapZ2	+	F-actin capping protein
Bid	+	Pro-apoptotic Bcl-2 family protein
Nip1	+	Nuclear cap binding protein
Phospho-p38	+	Active form of p38 MAP kinase, mediates stress related transcription/cell cycle control
MEK2	+	Dual specificity protein kinase, activates MAPK1/ERK2 and MAPK2/ERK3
Sema4c	+	Semaphorin, linked to differentiation and development
Caspase 7	−	Apoptotic caspase, activated by caspase 3 or caspase 10
Caspase 14	−	Apoptotic caspase, activated by caspase 8 or caspase 10
Rip2	−	Receptor interacting protein, has apoptosis inducing activity
Cdk2	−	Cyclin dependent kinase, essential for G1/S transition
RAS-GAP	−	GTPase activating protein, down regulates ras signaling
Rho-GDI	−	GDP dissociation inhibitor, stimulates signaling through Rho, a GTPase that regulates focal adhesions and stress fibers
ROCK-D1	−	Serine/threonine kinase, activated by Rho, a GTPase that regulates focal adhesions and stress fibers