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Brefeldin A blocks the long-term increase in Glu uptake. Synaptosomal Glu uptake was significantly increased 24 h after treatment of pleural-pedal ganglia with 5-HT in vitro (n = 7). Exposure to brefeldin A (18 μM) for 24 h, beginning immediately after the treatment blocked the increase in Glu uptake (n = 5). Brefeldin A treatment alone had no effect on Glu uptake (n = 5).
