Skip to main content
. Author manuscript; available in PMC: 2007 Jun 1.
Published in final edited form as: Vaccine. 2007 Jan 10;25(17):3437–3444. doi: 10.1016/j.vaccine.2006.12.045

Figure 2.

Figure 2

Protein Expression of the E7IR Vaccine. Cellular protein extracts from transfected NIH 3T3 cells were separated by SDS-PAGE and blotted onto nitrocellulose. Lanes are labeled as follows: lane 1 protein marker, lane 2 empty vector, lane 3 E7WT, and lane 4 E7IR vaccine construct. Panel A shows the 27kD E7IR protein detected with an HA-specific polyclonal antibody. Panel B shows detection of the E7WT protein, lane 3, 19kD and the E7IR protein, lane 4, 27kD with an HPV-16 E7 specific monoclonal antibody.