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. Author manuscript; available in PMC: 2007 Jun 1.
Published in final edited form as: Vaccine. 2007 Jan 10;25(17):3437–3444. doi: 10.1016/j.vaccine.2006.12.045

Figure 3.

Figure 3

Soft Agar Transformation Assay. Anchorage-independent growth was determined by the colony formation efficiency of cells suspended in soft agar. E7WT and E7IR transformed NIH 3T3 cells were plated in triplicate dilutions (1×104, 2×104, and 4×104) and colonies were stained and counted after a two week incubation. Mean colony counts were determined as a function of normalized triplicates in each dilution series. The E7IR construct is showing a background level of efficiency of transformation compared to the E7WT construct.