Skip to main content
. Author manuscript; available in PMC: 2007 Jun 1.
Published in final edited form as: Vaccine. 2007 Jan 10;25(17):3437–3444. doi: 10.1016/j.vaccine.2006.12.045

Figure 7.

Figure 7

In Vivo CTL. Five mice per group were vaccinated with the E7IR vaccine twice in a thirty day interval, CFSE-labeled target cells loaded with E7 (49-57) peptide or control peptide were injected into vaccinated mice. After 18 hours, splenocytes from vaccinated mice were harvested and flow cytometry was utilized to determine percentages of CFSElow and CFSEhigh target populations and were used to perform an in vivo cytotoxicity assay. Data were normalized compared to background lysis seen in naïve, non-tumor challenged mice. Administration of the E7IR vaccine caused significantly more lysis of target cells than administration of the wild type E7 gene (p=0.0001).