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. 2007 May 3;7:70. doi: 10.1186/1471-2148-7-70

Figure 2.

Figure 2

Western blot showing the expression of Trg1 in Heterosigma akashiwo. Total soluble proteins were separated on NuPAGE Novex Bis-Tris gel and probed with preadsorption control (lane 2), anti-Trg1 peptide antiserum, day 50 (lane 4) and preinjection antiserum, day 0 (lane 6). A band of the expected estimated size of 27 kDa is present in the anti-Trg1 peptide lane 4, but not in the two negative controls (preadsorption and day 0). Lanes 1, 3, and 5 contain "Magic Mark Western" protein standard.