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Result of nested multiplex PCR on representative liver abscess pus and urine specimen. The E. histolytica (EH) and Internal amplification control (IAC) bands are 439 and 305 bp respectively. Lane-1 and 4 are positive for E. histolytica DNA in liver abscess pus and urine specimen respectively; Lane-2, 3 and 5 are negative for E. histolytica DNA; Lane-M, 100 bp DNA ladder (Bangalore genei, Bangalore).
