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Complementation of E. coli groES and groEL mutations using the cloned operon from B. bacilliformis
| E. coli strain (relevant mutation) | Growth at restrictive temperaturea when transformed with the following plasmids: |
|||
|---|---|---|---|---|
| pCR2.1-TOPO | pGROESL-TOPO | pACYC177 | pGROESL177 | |
| NRK117 (groEL) | − | + | − | + |
| CG2244 (groES) | − | − | − | + |
Restrictive temperature of 44 (NRK117) and 47 °C(CG2244). All strains grew at a permissive temperature of 30 °C.
