Figure 3.

Cell-cycle analyses of CD4⁺ T cells treated with DC-HIL-Fc. (A) T cells (6 × 10⁶) were labeled with CFSE (1 μM) and then cultured in microwells precoated with anti-CD3 Ab (0.3 μg/mL) plus hIgG or DC-HIL-Fc (5 μg/mL). At the indicated time points, cells were harvested and analyzed by FACS for fluorescence intensity. The frequency (%) of divided cells is shown in histograms. (B) T cells from 48-hour culture similarly treated were analyzed for incorporation of BrdU (using FITC–anti-BrdU Ab) and total DNA content (stained with 7-AAD) by FACS; data shown as dot plots of BrdU versus 7-AAD. Data shown are representative of 3 (A) and 2 (B) independent experiments.