Table 1.

Oligo sequences

Gene product	Gene symbol	Forward primer	Reverse primer	Accession no.
CCAAT/enhancer binding protein-α	Cebpa	5′-agagccgagataagccaaa-3′	5′-ggtcaactccagcaccttct-3′	NM_007678
Ferritin heavy chain 1	Fth1	5′-aagaaaccagaccgtgatga-3′	5′-tagccagtttgtgcagttcc-3′	NM_010239
Ferritin light chain 1	Ftl1	5′-ctcatcaagaagatgggcaa-3′	5′-gagatactcgcccagagacc-3′	NM_010240
Hemochromatosis	Hfe	5′-gcaatctcaggccatgatta-3′	5′-attccaaccaagaagatggc-3′	NM_010424
Hemochromatosis type 2 (juvenile)	Hjv, Hfe2	5′-gctcgagtttgtccattcaa-3′	5′-tgttccaatgtaggcagctc-3′	NM_027126
Hepcidin antimicrobial peptide 1	Hamp, Hamp1	5′-tgagcagcaccacctatctc-3′	5′-acagcagaagatgcagatgg-3′	NM_032541
Solute carrier family 11	Dmt1, Slc11a2	5′-tgtttgattgcattgggtctg-3′	5′-cgctcagcaggactttcgag-3′	BC019137
Solute carrier family 40	Fpn1, Slc40a1	5′-caagaatgagctcctgacca-3′	5′-gccacaacaacaatccagtc-3′	NM_016917
Transferrin	Tf, Trf	5′-cttctcatgctgttgtggct-3′	5′-gttcctgtgccactttgaga-3′	NM_133977
Transferrin receptor	Tfr1, Tfrc	5′-cagaccttgcactctttgga-3′	5′-gaaagaaggaaagccaggtg-3′	NM_011638
Transferrin receptor 2	Tfr2, Trfr2	5′-gggttcagacctcgaagaag-3′	5′-aggctcacgtacacaacagc-3′	NM_015799
β-actin	Actb	5′-ccttccttcttgggtatgga-3′	5′-acggatgtcaacgtcacact-3′	NM_007393

Primer design was performed using Primer Express Software version 2.0 (Applied Biosystems), based on the corresponding mouse gene sequences obtained from PubMed. As endogenous controls to normalize gene expression levels, a mouse Gapdh VIC/MGB probe (Applied Biosystems) and mouse β-actin were used. Two endogenous control genes were used to determine if any difference in amplification existed due to the effect of iron overload.