Table 3.
Characteristics and assay results for 8 individuals with a history of variola virus infection.
| Assay result |
||||||||
|---|---|---|---|---|---|---|---|---|
| Individual (age in years, sex) | Years since variola virus infection | Facial scars, no. | VV vaccinationa | ELISPOT, sfu/106 PBMCs | CTL, LU | Lymphoproliferation, SI |
nAb titer | |
| Whole VV | Heat-inactivated VacAg | |||||||
| 1 (62, male) | 60 | ∼50 | - | 0 | NA | 5b | 5b | 1:80b |
| 2 (55, male) | 51 | 25 | - | 1.3 | 3.2 | 2.4 | 2 | 1:10 |
| 3 (69, male) | 63 | 25 | - | 1.3 | 0 | 1.3 | 16b | 1:40b |
| 4 (71, female) | 64 | 20 | - | 2.7 | 0 | 2 | 24b | 1:1280b |
| 5 (64, male) | 61 | ∼100 | - | 1.3 | .4 | 1 | 29b | 1:160b |
| 6 (78, female) | 77 | 0 | + | 0 | .7 | 153b | 131b | 1:80b |
| 7 (50, male) | 44 | ∼50 | + | 2.7 | 2.2 | 38b | 86b | 1:80b |
| 8 (61, female) | 45 | ∼50 | + | 25.3b | .9 | 20b | 8b | 1:320b |
NOTE. CTL, cytotoxic T lymphocyte; ELISPOT, enzyme-linked immunospot; LU, lytic unit; NA, not available; nAb, neutralizing antibody; PBMC, peripheral blood mononuclear cell; SI, stimulation index; VacAg, vaccinia antigen; VV, vaccinia virus.
a
A plus sign (+) indicates presence of a symptom, and a minus sign (-) indicates absence of a symptom.
b
Value exceeds positive cutoff level for assay.