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. 2007 May 14;104(21):8839–8844. doi: 10.1073/pnas.0611416104

Fig. 6.

Fig. 6.

Formation of a ternary complex among Hsp90, β-actin, and NOS-3 leads to a decrease in bound Hsp90. Platelets from healthy subjects were treated with cytochalasin D 5 μmol/liter or vehicle, after which they were lysed and, after immunoprecipitation of NOS-3 or β-actin, immunoprecipitates were run on SDS/PAGE and Western blotted for either NOS-3 (A) or β-actin (B). Typical blots are shown (V, vehicle; C, cytochalasin D treatment), and results are presented for band density, as determined by scanning densitometry (n = 6, ±SD). Cytochalasin D does not affect the degree of coimmunoprecipitation of NOS-3 and β-actin. (C) Cytochalasin D treatment decreases Hsp90 levels in NOS-3 and β-actin immunoprecipitates. Platelets from healthy subjects were treated with cytochalasin D or vehicle, after which they were lysed and, after immunoprecipitation of NOS-3 or β-actin, immunoprecipitates were run on SDS/PAGE and Western blotted for Hsp90. A typical blot is shown (V, vehicle; C, cytochalasin D treatment), and results are presented for band density, as determined by scanning densitometry (n = 6, ±SD, ∗, P < 0.01 for cytochalasin D- versus vehicle-treated platelets).