Table 1.

Detection of poison frog alkaloids of 11 structural classes in mite extracts

Site	Sample no.	Structural class																																				Unclassifed
		5,8-I													d-5,8-I			5,6,8-I									PTX			hPTX	1,4Q	4,6-Q	3,5-I	Pyr		Spiro	Tri
		195I	203A	205A	207A	209S	219F/L	223D	223V	225D	231C	235B″	237D	261D	205L	243F	269D	195G	223A	235E	237A	237C	237L	251T	253H	259C	251D	307F	307A	251R	233A	237I	223AB	183B	253I	236	193C	181C	209G	227	265K	279I	323I
Panama		○			●	○		○		○		●	○													○				○					○						○
Isla Escudo	1				●
	2																○																							○
Cayo Agua	1											●	●
	2											●															●
	3			●	●	○						●						○
Isla Popa	1
	2						●		●							●	●			●																		○
	3						●		●			●				●	●			●											●
	4											●	○																		●
	5											●	○
	6		●	●	●
Cerro Brujo	1											●	○
	2											●																●
	3											●
	4											●																●
	5																	○										●
Cayo Nancy	1																					●	●		●												○					●	●
	2											●							●																	●
Isla Bastimentos	1											●	○
	2																		●										●
	3											●
	4			●	●	○															●								●
	5																																									●	●
Mainland S. of Pastores	1																																									○	○
Costa Rica											●		●	●				●
Rio Sand Box	1	●				●				○
Roja Maca	1
Isais	1	●			●										●							●											●	●					●
La Selva	1											●
Tortuguero	1											●												●			●
	2																										●
	3																					○										○										○	○
	4																										●

The filled circles indicate the presence of alkaloids in extracts of mites and O. pumilio from the same site; open circles indicate the presence of alkaloids only in mite extracts from that site. Only alkaloids previously reported from poison frogs (see ref. 6) are included. For the mite alkaloids not reported from poison frogs, see SI Data Set 2. Three isomers of 207A were detected in mites (see SI Data Set 1). The identity of the 5,8-Is 219F and 219L could not be determined from the GC–MS data. PTX, pumiliotoxin; Pyr, pyrrolidine; Spiro, spiropyrrolizidine; Tri, tricyclic.