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GUS expression from minichromosome 86B23 with the pAHC25 transgene. sh, shoot; em, embryo; en, endosperm. (A) GUS expression in germinated seedling, root, and shoot (blue). (B and C) GUS expression in segregating mature kernels of a selfed plant with one copy of 86B23 minichromosome and one normal B chromosome. GUS expression is absent in kernel (B) but present in both embryo and endosperm of kernel (C). GUS expression was not found in the recipient HiII strain (not shown).
